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plasmid pcl eco  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pcl eco
    Plasmid Pcl Eco, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pcl+eco/pCI-neo-mOVA+(Plasmid+%2325099)/pm41730908-370-18-20
    Average 93 stars, based on 35 article reviews
    plasmid pcl eco - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
    Article Snippet: .. HEK293 cells were co-transfected with 4.75 μg of gag/pol/env-encoding plasmid pCL-Eco (Addgene #12371; http://n2t.net/addgene:12371 ; RRID:Addgene_12371, gift from Inder Verma) and 3 μg of murine retroviral constructs pMSCV, mCAR27, mCAR27/28, and mCAR27/BB using GeneJuice (#70967, Sigma-Aldrich). ..

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1 mg/mL in a 10 cm culture dish. ..

    Article Title: MLL5 is involved in retinal photoreceptor maturation through facilitating CRX-mediated photoreceptor gene transactivation
    Article Snippet: Plasmid: PMD2.G , Addgene , 12259. .. Plasmid: PCL-Eco , Addgene , 12371. .. Plasmid: VSV-G , Addgene , 138479.

    Article Title: Peptide-MHC-targeted retroviruses enable in vivo expansion and gene delivery to tumor-specific T cells
    Article Snippet: .. The plasmid pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371 ; http://n2t.net/addgene:12371 ; RRID:Addgene_12371) The plasmid pUMVC was a gift from Bob Weinberg (Addgene plasmid # 8449 ; http://n2t.net/addgene:8449 ; RRID:Addgene_8449). .. MSCV ZsGreen plasmid was cloned by inserting an IRES followed by the coding region of ZsGreen into the MSCV vector backbone, involving digestion with XhoI/EcoRI (NEB) and subsequent Gibson Assembly. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ; http://n2t.net/addgene:12259 ; RRID:Addgene_12259).

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells.
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1mg/mL in a 10 cmculture dish. ..

    Article Title: Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
    Article Snippet: Plasmid: pBabe-Ras , Gift from William Hahn ( viral vectors ) , Addgene 9051. .. Plasmid: pCL Eco , Naviaux et al. , Addgene 12371. .. Plasmid: pBabe-PURO-EGFP(PIG) , Gift from Scott Lowe ( Lowe Lab Plasmids ) , Addgene 18751.

    Article Title: Peptide-MHC–targeted retroviruses enable in vivo expansion and gene delivery to tumor-specific T cells
    Article Snippet: .. The plasmid pCL-Eco was a gift from I. Verma (Addgene, plasmid # 12371; http://n2t.net/addgene:12371 ; RRID:Addgene_12371). .. The plasmid pUMVC was a gift from B. Weinberg (Addgene, plasmid # 8449; http://n2t.net/addgene:8449 ; RRID:Addgene_8449).

    Article Title: The proprotein convertase furin regulates the development of thymic epithelial cells to ensure central immune tolerance
    Article Snippet: Plasmid: MSCV-PGK-Furin-IRES-GFP , This paper , N/A. .. Plasmid: pCL-Eco , Addgene , Cat# 12371; RRID:Addgene_12371. ..

    Retroviral:

    Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
    Article Snippet: .. HEK293 cells were co-transfected with 4.75 μg of gag/pol/env-encoding plasmid pCL-Eco (Addgene #12371; http://n2t.net/addgene:12371 ; RRID:Addgene_12371, gift from Inder Verma) and 3 μg of murine retroviral constructs pMSCV, mCAR27, mCAR27/28, and mCAR27/BB using GeneJuice (#70967, Sigma-Aldrich). ..

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1 mg/mL in a 10 cm culture dish. ..

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells.
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1mg/mL in a 10 cmculture dish. ..

    Construct:

    Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
    Article Snippet: .. HEK293 cells were co-transfected with 4.75 μg of gag/pol/env-encoding plasmid pCL-Eco (Addgene #12371; http://n2t.net/addgene:12371 ; RRID:Addgene_12371, gift from Inder Verma) and 3 μg of murine retroviral constructs pMSCV, mCAR27, mCAR27/28, and mCAR27/BB using GeneJuice (#70967, Sigma-Aldrich). ..

    Transduction:

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1 mg/mL in a 10 cm culture dish. ..

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells.
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1mg/mL in a 10 cmculture dish. ..

    Transfection:

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1 mg/mL in a 10 cm culture dish. ..

    Article Title: The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells.
    Article Snippet: .. For retroviral transduction of primary cells, Phoenix packaging cells (ATCC, #CRL-3214) were transfected with 20 μg of the plasmid pCL-Eco (Addgene, #25099) and 16 μg of pMXs-EHF, or the empty vector pMXs, with 90 μL of the transfecting reagent polyethylenimine (Polysciences, #23966-1) at 1mg/mL in a 10 cmculture dish. ..



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